Garrick R A, Redwood W R
Am J Physiol. 1977 Sep;233(3):C104-10. doi: 10.1152/ajpcell.1977.233.3.C104.
Mixtures of viable endothelial and epithelial cells were separated by enzymatic digestion from rabbit lung and recovered by centrifugation. The cells were mixed with an extracellular marker and packed by centrifugation into small-diameter polyethylene tubing and pulsed with tritiated water and 14C-labeled alcohols. Calculation of diffusion coefficients for the packed cell column (D), intracellular material (D2), and extracellular fluid (D1) was based on a local steady-state one-dimensional diffusional model. Permeability coefficients were: tritiated water, 288 X 10(-5) cm s-1; methanol, 385 X 10(-5) cm s-1; ethanol, 214 X 10(-5) cm s-1; propanol, 277 X 10(-5) cm s-1; and hexanol, 1255 X 10(-5) cm s-1. The permeability coefficients of these aliphatic alcohols show a minimum at ethanol with hexanol having the highest value of all substances tested. The results support the concept of parallel aqueous and lipid pathways for small solutes in the plasma membrane. Study of the permeability properties of isolated lung cells can provide information on the cellular pathway in the transcapillary transport of water and solutes in the lung.
通过酶消化从兔肺中分离出有活力的内皮细胞和上皮细胞混合物，并通过离心回收。将细胞与细胞外标记物混合，通过离心装入小直径聚乙烯管中，并用氚水和14C标记的醇进行脉冲处理。基于局部稳态一维扩散模型计算填充细胞柱（D）、细胞内物质（D2）和细胞外液（D1）的扩散系数。渗透系数分别为：氚水，288×10(-5) cm s-1；甲醇，385×10(-5) cm s-1；乙醇，214×10(-5) cm s-1；丙醇，277×10(-5) cm s-1；己醇，1255×10(-5) cm s-1。这些脂肪醇的渗透系数在乙醇处显示出最小值，己醇在所有测试物质中具有最高值。结果支持质膜中小溶质的平行水相和脂质途径的概念。对分离的肺细胞渗透特性的研究可以提供关于肺中毛细血管跨膜水和溶质转运的细胞途径的信息。
