Tron F, Le Guern C, Cazenave P A, Bach J F
Eur J Immunol. 1982 Sep;12(9):761-6. doi: 10.1002/eji.1830120911.
Immunization of NZB and A/J mice against an anti-DNA hybridoma antibody (F227) derived from (NZB x NZW)F1 (B/W) mice allowed the preparation of two anti-idiotypic antisera. These two reagents were shown to recognize different idiotopes of the F227 monoclonal antibody. NZB anti-idiotypic antibodies recognized non-ligand-modifiable idiotypic determinants. These idiotopes were private or present at undetectable level in BW mouse sera since it was found that only two of the 24 B/W mouse sera tested were recognized by these antibodies. Conversely, A/J anti-idiotypic antibodies recognized partially ligand-modifiable idiotopes which were found in all B/W mouse sera tested. These results demonstrate that anti-DNA antibodies share similar idiotypic specificities and suggest that these autoantibodies occur as families of structurally related proteins.
用源自（新西兰黑鼠×新西兰白鼠）F1（B/W）小鼠的抗DNA杂交瘤抗体（F227）对新西兰黑鼠和A/J小鼠进行免疫，从而制备了两种抗独特型抗血清。结果表明，这两种试剂可识别F227单克隆抗体的不同独特型。新西兰黑鼠抗独特型抗体识别不可被配体修饰的独特型决定簇。这些独特型是个体特有的，或在BW小鼠血清中以不可检测的水平存在，因为发现所检测的24份B/W小鼠血清中只有两份能被这些抗体识别。相反，A/J抗独特型抗体识别部分可被配体修饰的独特型，在所检测的所有B/W小鼠血清中都能发现这些独特型。这些结果表明，抗DNA抗体具有相似的独特型特异性，并提示这些自身抗体以结构相关蛋白家族的形式出现。
