Laurila P, Stenman S
Exp Cell Res. 1982 Nov;142(1):15-23. doi: 10.1016/0014-4827(82)90403-7.
The pericellular fibronectin matrix of human fibroblasts is lost when they are fused with normal or malignant cells, which do not produce fibronectin matrix. In the present study we have investigated whether also the cytoplasmic fraction of fibronectin-negative HeLa or MDCK cells can cause this effect. Enucleated epithelial cells (cytoplasts) were therefore fused with norman human fibroblasts. Fibronectin expression of the resulting cytoplasmic hybrids (cybrids) was studied by the indirect immunofluorescence technique. Three hours after fusion cybrids formed between fibroblasts and enucleated epithelial cells showed fibronectin matrix expression clearly weaker than that seen on the intact fibroblasts. An accumulation of fibronectin matrix was observed in the cybrids, analogously to the intact fibroblasts, and already 12-24 h after fusion the cybrids showed a fibronectin matrix expression similar to that of the fibroblasts. No fibronectin matrix was detected in the epithelial cells or their cytoplast. Our results indicate that cytoplasmic factors from epithelial cells are able to cause an initial suppression in the formation of fibronectin matrix. However, the cytoplasts are not capable of causing a long-term effect on the fibroblasts.
当人成纤维细胞与不产生纤连蛋白基质的正常细胞或恶性细胞融合时，其细胞周围的纤连蛋白基质会丧失。在本研究中，我们调查了纤连蛋白阴性的HeLa细胞或MDCK细胞的细胞质部分是否也会产生这种效应。因此，将去核的上皮细胞（胞质体）与正常人成纤维细胞融合。通过间接免疫荧光技术研究所得细胞质杂种细胞（胞质杂合体）的纤连蛋白表达。成纤维细胞与去核上皮细胞融合3小时后，形成的胞质杂合体显示出的纤连蛋白基质表达明显弱于完整成纤维细胞上的表达。与完整的成纤维细胞类似，在胞质杂合体中观察到纤连蛋白基质的积累，并且在融合后12 - 24小时，胞质杂合体显示出与成纤维细胞相似的纤连蛋白基质表达。在上皮细胞或其胞质体中未检测到纤连蛋白基质。我们的结果表明，来自上皮细胞的细胞质因子能够对纤连蛋白基质的形成产生初始抑制作用。然而，胞质体对成纤维细胞不能产生长期影响。
