胃液中的总亚硝基化合物
Total nitroso compounds in gastric juice.
作者信息
Bavin P M, Darkin D W, Viney N J
出版信息
IARC Sci Publ. 1982(41):337-44.
A method is described for the measurement of 'total nitroso compounds' in gastric juice. It is applicable to small samples and in the presence of food. The method depends on treatment with hydrazine sulfate at pH 4 to destroy nitrite, decomposition by addition of the sample to HBr/acetic acid in boiling ethyl acetate and determination of the nitric oxide evolved by a chemiluminescence analyser. The method is being applied to a study of samples from healthy subjects throughout 24 hours of normal diet, prior to similar studies with patients.
描述了一种测定胃液中“总亚硝基化合物”的方法。该方法适用于少量样品以及存在食物的情况。该方法基于在pH 4条件下用硫酸肼处理以破坏亚硝酸盐，将样品加入沸腾的乙酸乙酯中的氢溴酸/乙酸中进行分解，并通过化学发光分析仪测定释放出的一氧化氮。该方法正在应用于对正常饮食24小时的健康受试者的样品进行研究，之后再对患者进行类似研究。
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