Moore W E, Holdeman L V, Smibert R M, Good I J, Burmeister J A, Palcanis K G, Ranney R R
Infect Immun. 1982 Nov;38(2):651-67. doi: 10.1128/iai.38.2.651-667.1982.
From replicate trials of experimental gingivitis in four periodontally healthy subjects, 166 bacterial species and subspecies were detected among 3,034 randomly selected isolates from 96 samples. Of these bacteria, Actinomyces naeslundii (serotype III and phenotypically similar strains that were unreactive with available antisera), Actinomyces odontolyticus (serotype I and phenotypically similar strains that were unreactive with available antisera), Fusobacterium nucleatum, Lactobacillus species D-2, Streptococcus anginosus, Veillonella parvula, and Treponema species A appeared to be the most likely etiological agents of gingivitis. Statistical interpretations indicated that the greatest source of microbiological variation of the total flora observed was person-to-person differences in the floras. The next greatest source of variation was the inflammatory status of the sample sites. Person-to-person differences were smallest at experimental day 4. The floras became more diverse with time and as gingivitis developed and progressed. Analyses indicated that sequential colonization by certain species was repeatable and therefore probably predictable. Variation was relatively small between replicate trials, between two sites on the same teeth sampled on the same day, and between the same sites sampled at the same relative time in a replicate trial.
在对四名牙周健康受试者进行的实验性牙龈炎重复试验中，从96个样本的3034株随机选择的分离株中检测到166种细菌物种和亚种。在这些细菌中，内氏放线菌（血清型III以及与现有抗血清无反应的表型相似菌株）、溶齿放线菌（血清型I以及与现有抗血清无反应的表型相似菌株）、具核梭杆菌、乳酸杆菌D-2种、咽峡炎链球菌、小韦荣球菌和密螺旋体A种似乎是牙龈炎最可能的病原体。统计分析表明，观察到的总菌群微生物变异的最大来源是菌群的个体差异。第二大变异来源是样本部位的炎症状态。个体差异在实验第4天时最小。随着时间推移以及牙龈炎的发展和进展，菌群变得更加多样化。分析表明，某些物种的顺序定殖是可重复的，因此可能是可预测的。在重复试验之间、同一天在同一颗牙齿上采集的两个部位之间以及重复试验中在相同相对时间采集的相同部位之间，变异相对较小。
