Moore W E, Holdeman L V, Smibert R M, Hash D E, Burmeister J A, Ranney R R
Infect Immun. 1982 Dec;38(3):1137-48. doi: 10.1128/iai.38.3.1137-1148.1982.
A total of 78 bacteriological samples were taken from the supragingival tooth surface after superficial cleaning with toothpicks or from the periodontal sulci of 42 affected sites in 21 adolescents or young adults with severe generalized periodontitis. Of 190 bacterial species, subspecies, or serotypes detected among 2,723 isolates, 11 species exceeded 1% of the subgingival flora and were most closely associated with the diseased sulci. Eleven others were also sufficiently frequent to be suspect agents of tissue destruction. Many of these species are known pathogens of other body sites. In addition, 10 species of Treponema were isolated. One of these and the "large treponeme" were also more closely associated with severe periodontitis than they were with healthy sites or gingivitis. There were highly significant differences between the composition of the flora of the affected sulci and the flora of (i) the adjacent supragingival tooth surface, (ii) the gingival crevice of periodontally healthy people, and (iii) sites with a gingival index score of 0 or 2 in experimental gingivitis studies. The floras of different individuals were also significantly different. There was no statistically detectable effect of sampling per se upon the composition of the flora of subsequent samples from the same sites. The composition of the supragingival flora of the patients with severe generalized periodontitis that had serum antibody to Actinobacillus actinomycetemcomitans was significantly different from the supragingival flora of patients without this serum antibody. However, there was no statistically significant difference in the composition of their subgingival floras.
在21名患有重度广泛性牙周炎的青少年或年轻成年人中，用牙签进行表面清洁后，从龈上牙表面或42个患部的牙周袋中总共采集了78份细菌学样本。在2723株分离菌中检测到190种细菌、亚种或血清型，其中11种在龈下菌群中所占比例超过1%，且与患病牙周袋关系最为密切。另外11种出现频率也足够高，被怀疑是组织破坏的病原体。这些物种中有许多是其他身体部位已知的病原体。此外，还分离出10种密螺旋体。其中一种和“大密螺旋体”与重度牙周炎的关联也比与健康部位或牙龈炎的关联更为密切。患部牙周袋菌群的组成与（i）相邻龈上牙表面、（ii）牙周健康者的龈沟以及（iii）实验性牙龈炎研究中牙龈指数评分为0或2的部位的菌群组成之间存在高度显著差异。不同个体的菌群也存在显著差异。采样本身对来自同一部位后续样本的菌群组成没有统计学上可检测到的影响。患有重度广泛性牙周炎且血清中含有伴放线放线杆菌抗体的患者，其龈上菌群组成与没有这种血清抗体的患者的龈上菌群组成有显著差异。然而，他们龈下菌群的组成没有统计学上的显著差异。
