Casellas P, Brown J P, Gros O, Gros P, Hellström I, Jansen F K, Poncelet P, Roncucci R, Vidal H, Hellström K E
Int J Cancer. 1982 Oct 15;30(4):437-43. doi: 10.1002/ijc.2910300410.
Conjugates (immunotoxins) comprising ricin A-chain and monoclonal antibody 96.5, which is specific for human melanoma-associated antigen p97, inhibited protein synthesis and colony formation of cultured human melanoma cells that expressed more than 80,000 molecules of p97 per cell. Cells expressing fewer than 5,000 molecules of p97 were not killed. The presence of 10 mM ammonium chloride significantly increased the efficiency of the immunotoxin, tumor cells expressing high levels of p97 being killed at immunotoxin concentrations as low as 10(-10) M.
由蓖麻毒素A链和单克隆抗体96.5组成的偶联物（免疫毒素），对人黑色素瘤相关抗原p97具有特异性，可抑制每细胞表达超过80,000个p97分子的培养人黑色素瘤细胞的蛋白质合成和集落形成。表达少于5,000个p97分子的细胞未被杀死。10 mM氯化铵的存在显著提高了免疫毒素的效率，表达高水平p97的肿瘤细胞在低至10(-10) M的免疫毒素浓度下被杀死。
