Brihaye C, Guillaume M, Lavi N, Cogneau M
J Nucl Med. 1982 Dec;23(12):1114-20.
A new mercury-195m (T1/2: 41 hr) leads to gold-195m (T1/2: 30.6 sec) generator suitable for first-pass as well as steady-state radionuclide angiography studies has been developed. The distribution coefficients, Kd, for mercury (Hg-195m) and gold (Au-196), experimentally evaluated on chelating resin Chelex 100, showed this resin to be suitable as the column packing. Mercury-195m in a Trisma buffer solution at pH 7.40 was loaded on a chelating resin Chelex 100 column, together with gold carrier. Au-195m was eluted with an aqueous solution of 5% glucose in a 0.01M Trisma buffer at pH 7.70 (25 degrees C). Chemical assessment of the eluate has ensured that the eluted carrier-free gold is by no means colloidal: 80% is found in an ionic form, 20% as an uncharged species. Safe clinical use for routine diagnosis in humans is possible.
一种新型的汞 - 195m（半衰期：41小时）到金 - 195m（半衰期：30.6秒）的发生器已被开发出来，适用于首次通过以及稳态放射性核素血管造影研究。在螯合树脂Chelex 100上通过实验评估得到的汞（Hg - 195m）和金（Au - 196）的分配系数Kd表明，这种树脂适合作为柱填充物。将pH值为7.40的Trisma缓冲溶液中的汞 - 195m与金载体一起加载到螯合树脂Chelex 100柱上。在pH值为7.70（25摄氏度）的0.01M Trisma缓冲溶液中，用5%葡萄糖水溶液洗脱金 - 195m。对洗脱液的化学评估确保了洗脱的无载体金绝不是胶体形式：80%以离子形式存在，20%为不带电物种。这种发生器可安全地用于人体常规诊断。
