Iushchuk N D, Likhoded V G, Iakovlev M Iu, Kudriavtsev A E, Apollonin A V, Kozlova N N
Zh Mikrobiol Epidemiol Immunobiol. 1996 Nov-Dec(6):50-3.
The state of immunity to endotoxin of gram-negative bacteria in 45 patients with purulent meningitis caused by meningococci and Escherichia was studied. For comparison, similar characteristics in 35 practically healthy persons were studied. The state of immunity was evaluated by antibody titers in the passive hemagglutination inhibition test with chemotype Re glycolipid and by the content of polymorphonuclear leukocytes, capable of binding endotoxin in the blood of the examinee (in vivo determination), and leukocytes, capable of binding endotoxin during the treatment of thin blood smears (in vitro determination). Leukocytes bound with endotoxin were detected in blood smears in the enzyme immunoassay with the use of the conjugate of horse-radish peroxidase with antibodies to chemotype Re glycolipid. The study revealed that the acute period of bacterial meningitis was characterized by considerable suppression of antiendotoxin immunity. Leukocytes, binding endotoxin in vivo, as well as leukocytes, capable of binding endotoxin in vitro, were practically absent in the patients at the time of their admittance to the hospital. After treatment the characteristics of antiendotoxin immunity restored practically to normal values.
研究了45例由脑膜炎球菌和大肠杆菌引起的化脓性脑膜炎患者对革兰氏阴性菌内毒素的免疫状态。为作比较，研究了35名实际健康者的类似特征。免疫状态通过用Re化学型糖脂进行被动血凝抑制试验中的抗体滴度、受检者血液中能够结合内毒素的多形核白细胞含量（体内测定）以及在薄血涂片处理过程中能够结合内毒素的白细胞（体外测定）来评估。在用辣根过氧化物酶与Re化学型糖脂抗体的缀合物进行酶免疫测定的血涂片中检测到与内毒素结合的白细胞。研究发现，细菌性脑膜炎急性期的特征是抗内毒素免疫受到明显抑制。患者入院时，体内结合内毒素的白细胞以及体外能够结合内毒素的白细胞实际上不存在。治疗后，抗内毒素免疫特征实际上恢复到正常水平。
