Sebunya T N, Saunders J R
Am J Vet Res. 1982 Oct;43(10):1793-8.
A murine model of experimentally induced Haemophilus pleuropneumoniae respiratory tract infections was developed and used to evaluate the efficacy of bacterins. Various doses of 3 H pleuropneumoniae strains (serotype 1 and 5) were administered intranasally (IN) to groups of anesthetized Swiss White mice. The accumulated mortality per group at 5 days after they were inoculated was used to calculate the median lethal dose (LD50), which ranged between 1.4 x 10(6) and 5.4 x 10(7) bacteria. There was correlation between dose administered, incubation period of the disease, and mortality. The induction of H pleuropneumoniae disease in the mouse was reproducible and resembled that of naturally occurring porcine Haemophilus pleuropneumonia. The murine model of exposure was used to evaluate the efficacy of H pleuropneumoniae serotype 5 (strain B78-3760) bacterins administered subcutaneously (SC) and IN. Vaccination by SC route induced slightly higher titers of specific seroagglutinins, compared with the response induced by the IN route, and the former was more effective in protecting mice against IN challenge with H pleuropneumoniae. However, vaccination was not efficacious in mice given a challenge dose greater than 1.2 LD50. The percentage survival rates in the SC and IN vaccinated mice were significantly greater than in the control groups at P less than 0.01 and P less than 0.05, respectively.
建立了实验性诱导猪胸膜肺炎放线杆菌呼吸道感染的小鼠模型，并用于评估菌苗的效力。将不同剂量的3株猪胸膜肺炎放线杆菌菌株（血清型1和5）经鼻内（IN）接种给麻醉的瑞士小白鼠组。接种后5天每组的累积死亡率用于计算半数致死剂量（LD50），其范围在1.4×10⁶至5.4×10⁷个细菌之间。给药剂量、疾病潜伏期和死亡率之间存在相关性。小鼠中猪胸膜肺炎放线杆菌疾病的诱导是可重复的，并且类似于自然发生的猪胸膜肺炎。该暴露小鼠模型用于评估经皮下（SC）和经鼻内接种的猪胸膜肺炎放线杆菌血清型5（菌株B78 - 3760）菌苗的效力。与经鼻内途径诱导的反应相比，经皮下途径接种诱导的特异性血清凝集素滴度略高，并且前者在保护小鼠免受猪胸膜肺炎放线杆菌经鼻内攻击方面更有效。然而，对于给予大于1.2 LD50攻击剂量的小鼠，接种无效。皮下和经鼻内接种疫苗的小鼠的存活率百分比分别在P小于0.01和P小于0.05时显著高于对照组。
