Paulson D J, Shug A L
Basic Res Cardiol. 1982 Jul-Aug;77(4):460-3. doi: 10.1007/BF02005345.
We investigated the effect of L-carnitine on the recovery of cardiac function after ischemic arrest in the perfused rat heart. L-carnitine was added to a cardioplegic solution, both as a free base and hydrochloride. The addition of L-carnitine as a free base to the solution had no effect on recovery of cardiac function. When L-carnitine HCl was added to the cardioplegic solution, it was necessary to adjust the pH of the solution to 7.4. The hearts arrested with this solution showed a greater incidence of reperfusion dysrhythmias than those in the control or the free base solution, but the overall recovery of cardiac function was the same as control. The hydrochloride of L-carnitine is strongly acidic, and these findings indicate that either the free base or a properly buffered solution must be used to study effects of carnitine upon cardiac function.
我们研究了左旋肉碱对灌注大鼠心脏缺血停搏后心功能恢复的影响。左旋肉碱以游离碱和盐酸盐的形式添加到心脏停搏液中。向溶液中添加游离碱形式的左旋肉碱对心功能恢复没有影响。当将盐酸左旋肉碱添加到心脏停搏液中时，有必要将溶液的pH值调节至7.4。用该溶液停搏的心脏与对照组或游离碱溶液组相比，再灌注心律失常的发生率更高，但心功能的总体恢复情况与对照组相同。左旋肉碱盐酸盐呈强酸性，这些发现表明，必须使用游离碱或适当缓冲的溶液来研究肉碱对心功能的影响。
