Lyons S F, Schoub B D, Chiu M N, Crespi M
J Virol Methods. 1982 Oct;5(2):93-100. doi: 10.1016/0166-0934(82)90086-6.
An indirect radioimmunoassay for testing the antiviral activity of interferon (IFN) is described. Vero cells are seeded in microtitre plates, treated with appropriate dilutions of interferon and challenged with Sindbis virus. Viral yield is measured using specific antibody and radiolabelled protein A. The assay is able to detect IFN levels of 5 international units (I.U.)/ml, has a high degree of reproducibility, and could be easily adapted to various cell and virus combinations. This microsystem is technically simple, allows testing of small volumes of test material, and eliminates subjectivity in reading of end-points.
本文描述了一种用于检测干扰素（IFN）抗病毒活性的间接放射免疫测定法。将非洲绿猴肾细胞（Vero细胞）接种于微量滴定板中，用适当稀释的干扰素处理，然后用辛德毕斯病毒攻击。使用特异性抗体和放射性标记的蛋白A测量病毒产量。该测定法能够检测出5国际单位（I.U.）/毫升的干扰素水平，具有高度的可重复性，并且可以很容易地适用于各种细胞和病毒组合。这种微系统技术简单，允许检测少量的测试材料，并消除了终点读数的主观性。
