Scott J V, Choppin P W
J Virol Methods. 1982 Nov;5(3-4):173-9. doi: 10.1016/0166-0934(82)90007-6.
Conditions for increasing the yield of measles virus from cultured cells have been investigated. Important factors for high virus yields were found to be cell type, incubation at 33 degrees C, concentration of serum in the medium, use of virus as inoculum within five passages of plaque purification, and repeated harvesting of the virus-containing medium at 12 h intervals after extensive virus-induced cell fusion had occurred. Under these conditions, Vero cells infected with the Edmonston strain of measles virus yielded a total of 2.98 X 10(9) plaque-forming units (p.f.u.) of released virus, which is equivalent to 270 p.f.u./cell. A similar amount of cell-associated virus was produced.
已对提高培养细胞中麻疹病毒产量的条件进行了研究。发现高病毒产量的重要因素包括细胞类型、在33摄氏度下孵育、培养基中血清的浓度、在空斑纯化的五代内使用病毒作为接种物，以及在广泛的病毒诱导细胞融合发生后，每隔12小时重复收集含病毒的培养基。在这些条件下，用麻疹病毒埃德蒙斯顿株感染的非洲绿猴肾细胞产生了总共2.98×10⁹个释放病毒的空斑形成单位（p.f.u.），相当于每个细胞270个p.f.u.。产生了类似数量的细胞相关病毒。
