Haspel M V, Knight P R, Duff R G, Rapp F
J Virol. 1973 Oct;12(4):690-5. doi: 10.1128/JVI.12.4.690-695.1973.
The characteristics of infectious measles virus released from latently infected hamster embryo fibroblast cells are described. Low levels of virus were released spontaneously when the cultures were incubated at 37 C; this phenomenon was observed 19 passages after the cells had been exposed to the virus and has continued through cell passage 45. The virus yield could be significantly increased by cocultivation of the hamster cells with BSC-1 cells or incubation of the latently infected cells at 33.5 C rather than at 37 C. Measles virus released after cocultivation demonstrated increased cytopathology in cell culture and reduced temperature sensitivity when compared to the virus released at 33.5 C. After cell passage 45, there was an increase in spontaneous release of virus. However, the viruses recovered by cocultivation or temperature release after cell passage 45 were nearly identical. These observations suggest a possible mechanism for measles virus activation in cells latently infected with this virus.
本文描述了从潜伏感染的仓鼠胚胎成纤维细胞中释放出的传染性麻疹病毒的特性。当培养物在37℃孵育时，会自发释放少量病毒；在细胞接触病毒后的第19代观察到这种现象，并持续到第45代细胞传代。通过将仓鼠细胞与BSC-1细胞共培养，或将潜伏感染的细胞在33.5℃而非37℃孵育，可显著提高病毒产量。与在33.5℃释放的病毒相比，共培养后释放的麻疹病毒在细胞培养中表现出增强的细胞病变效应，且温度敏感性降低。在第45代细胞传代后，病毒的自发释放有所增加。然而，通过共培养或在第45代细胞传代后通过温度释放回收的病毒几乎相同。这些观察结果提示了一种该病毒潜伏感染细胞中麻疹病毒激活的可能机制。
