通过免疫沉淀法鉴定风疹病毒结构蛋白

Identification of rubella virus structural proteins by immunoprecipitation.

作者信息

Trudel M, Nadon F, Comtois R, Payment P, Bonneau A M, Lecomte J

出版信息

J Virol Methods. 1982 Nov;5(3-4):191-7. doi: 10.1016/0166-0934(82)90009-x.

Abstract

Immunoprecipitation of [3H]amino acid labelled virus with monoclonal or human convalescent rubella sera and subsequent analysis by electrophoresis and fluorography, revealed three structural proteins of rubella virus: VP3: 59,000; VP2: 44,800; and VP1: 33,000.

摘要

用单克隆抗体或人风疹康复血清对[³H]氨基酸标记的病毒进行免疫沉淀，随后通过电泳和荧光自显影分析，揭示了风疹病毒的三种结构蛋白：VP3：59,000；VP2：44,800；以及VP1：33,000。