Waxham M N, Wolinsky J S
Virology. 1985 May;143(1):153-65. doi: 10.1016/0042-6822(85)90104-7.
A panel of murine monoclonal antibodies prepared against rubella virus is described. Fourteen of these monoclonal antibodies react with the E1 glycoprotein of rubella virus and define a total of six spacially separate epitopes in competitive inhibition assays. Antibodies binding to epitopes E1(a), E1(b), E1(c), or E1(e) inhibit the hemagglutinin function of the virus, while antibodies binding to epitopes E1(d) or E1(f) do not. Monoclonal antibodies binding to epitopes E1(c) or E1(d) prevent virus infectivity and identify antigen in distinct intracytoplasmic vacuoles of rubella virus-infected Vero cells by indirect immunofluorescence. Monoclonal antibody to epitope E1(f) localizes antigen primarily to the plasma membrane of infected cells, while antibodies binding to epitopes E1(a), E1(b), or E1(e) localize antigen throughout the infected cell's cytoplasm. A single monoclonal antibody is described which only reacts with the mature form of the virion E2 glycoprotein after rubella virus is treated with a disulfide-bond reducing agent. This antibody immunoprecipitates a 43,000 MW precursor to the E2 glycoprotein from lysates of infected cells and localizes its antigen throughout the cytoplasm of infected cells. The five remaining monoclonal antibodies react with the rubella virus C polypeptide. They define four topographically separate epitopes on the C polypeptide, C(a), C(b), C(c), and C(d), each of which is diffusely distributed throughout the cytoplasm of rubella virus-infected cells.
本文描述了一组针对风疹病毒制备的鼠单克隆抗体。其中14种单克隆抗体与风疹病毒的E1糖蛋白反应，并在竞争抑制试验中确定了总共6个空间上分离的表位。结合表位E1(a)、E1(b)、E1(c)或E1(e)的抗体抑制病毒的血凝素功能，而结合表位E1(d)或E1(f)的抗体则不抑制。结合表位E1(c)或E1(d)的单克隆抗体可阻止病毒感染，并通过间接免疫荧光在风疹病毒感染的Vero细胞的不同胞质空泡中识别抗原。针对表位E1(f)的单克隆抗体主要将抗原定位在感染细胞的质膜上，而结合表位E1(a)、E1(b)或E1(e)的抗体则将抗原定位在整个感染细胞的细胞质中。描述了一种单克隆抗体，在用二硫键还原剂处理风疹病毒后，该抗体仅与病毒粒子E2糖蛋白的成熟形式反应。该抗体从感染细胞的裂解物中免疫沉淀出E2糖蛋白的43,000 MW前体，并将其抗原定位在感染细胞的整个细胞质中。其余5种单克隆抗体与风疹病毒C多肽反应。它们在C多肽上确定了4个拓扑上分离的表位，即C(a)、C(b)、C(c)和C(d)，每个表位都弥漫分布在风疹病毒感染细胞的细胞质中。
