Li Rui, Zhang Zhou-Tian-Le, Wang Yun, Jiang Gang-Qiang, Yin Min, Li Yu, Zhu Wen-Yong, Tang Shu-Kun
Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
School of Medicine, Yunnan University, Kunming, 650091, People's Republic of China.
Folia Microbiol (Praha). 2023 Feb;68(1):115-120. doi: 10.1007/s12223-021-00931-8. Epub 2022 Aug 17.
A Gram stain-positive, rod-shaped, motile, aerobic and terminal endospore formation bacterium, designated YIM B00362, was isolated from saline soil samples collected from a salt lake in Xinjiang Province, north-west China. Phylogenetic analysis based on the 16S rRNA gene sequences and whole genomes indicated that the isolate belongs to the genus Paenibacillus. However, the highest sequence similarity between strain YIM B00362 and the relatives was only 94.4%. Moreover, the DNA-DNA relatedness and ANI values between the novel isolate and the relative type strain, Paenibacillus antri SYSU K30003 was 13.6% and 70.3%, respectively. The major cellular fatty acids were anteiso-C, C and the major quinone was MK-7. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylglyceride, and two unidentified polar lipids. The genomic DNA G + C content was 50.9 mol%. The major whole-cell sugars contained glucose and galactose. On the basis of physiological, phenotypic, and chemotaxonomic data, strain YIM B00362 represents a novel species of genus Paenibacillus, for which the name Paenibacillus alkalitolerans sp. nov. is proposed. The type strain is YIM B00362 (= KCTC 43272 = CGMCC 1.18801 = NBRC 114667).
从中国西北部新疆某盐湖采集的盐土样本中分离出一株革兰氏阳性、杆状、具运动性、需氧且形成末端芽孢的细菌，命名为YIM B00362。基于16S rRNA基因序列和全基因组的系统发育分析表明，该分离株属于芽孢杆菌属。然而，菌株YIM B00362与其亲缘关系最近的菌株之间的最高序列相似性仅为94.4%。此外，该新分离株与亲缘关系最近的模式菌株——炭疽芽孢杆菌SYSU K30003的DNA-DNA杂交率和平均核苷酸一致性（ANI）值分别为13.6%和70.3%。主要细胞脂肪酸为anteiso-C、C，主要醌类为MK-7。该分离株含有内消旋二氨基庚二酸作为诊断性二氨基酸，主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油酯和两种未鉴定的极性脂。基因组DNA的G+C含量为50.9 mol%。主要全细胞糖包括葡萄糖和半乳糖。基于生理、表型和化学分类学数据，菌株YIM B00362代表芽孢杆菌属的一个新物种，为此提出名为耐碱芽孢杆菌（Paenibacillus alkalitolerans sp. nov.）。模式菌株为YIM B00362（=KCTC 43272=CGMCC 1.18801=NBRC 114667）。
