Stockman L, Roberts G D
J Clin Microbiol. 1982 Nov;16(5):965-7. doi: 10.1128/jcm.16.5.965-967.1982.
An enzymatic method involving a protease (pronase) for the elimination of interference factors in the latex test for cryptococcal antigen was developed and compared with dithiothreitol treatment. The two were equivalent in their ability to remove interference factors; however, the enzymatic method generally yielded higher titers. The method is simple, requires only 20 min, and makes the latex test for cryptococcal antigen specific.
开发了一种涉及蛋白酶(链霉蛋白酶)的酶法,用于消除乳胶法检测隐球菌抗原中的干扰因素,并与二硫苏糖醇处理方法进行了比较。两种方法去除干扰因素的能力相当;然而,酶法通常能产生更高的滴度。该方法简单,仅需20分钟,且能使乳胶法检测隐球菌抗原具有特异性。