使用链霉蛋白酶消除隐球菌抗原乳胶凝集试验中干扰因素的经验。
Experience with the use of pronase to eliminate interference factors in the latex agglutination test for cryptococcal antigen.
作者信息
Gray L D, Roberts G D
机构信息
Department of Laboratory Medicine and Pathology, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
出版信息
J Clin Microbiol. 1988 Nov;26(11):2450-1. doi: 10.1128/jcm.26.11.2450-2451.1988.
Abstract
Cryptococcal antigen titers of 70 cerebrospinal fluid and 57 serum specimens from patients suspected of having cryptococcosis were determined both before and after treatment with pronase. Median titers of cerebrospinal fluid specimens before and after treatment were 128 and 128, respectively; mean geometric titers of these specimens before and after treatment were 102 and 204, respectively. Median titers of the serum specimens before and after treatment were 16 and 512, respectively; mean geometric titers of these specimens before and after treatment were 19 and 631, respectively. The modified latex agglutination test did not detect antigen in any of 50 cerebrospinal fluid and 51 serum specimens from patients not suspected of having cryptococcosis. These results suggest that the pronase modification increases the sensitivity of the latex agglutination test and that the modification be routinely incorporated into it.
摘要
对70份疑似患有隐球菌病患者的脑脊液标本和57份血清标本，在使用链霉蛋白酶治疗前后均测定了隐球菌抗原滴度。治疗前后脑脊液标本的中位滴度分别为128和128；这些标本治疗前后的几何平均滴度分别为102和204。治疗前后血清标本的中位滴度分别为16和512；这些标本治疗前后的几何平均滴度分别为19和631。改良乳胶凝集试验在50份非疑似患有隐球菌病患者的脑脊液标本和51份血清标本中均未检测到抗原。这些结果表明，链霉蛋白酶改良法提高了乳胶凝集试验的灵敏度，且该改良法应常规纳入该试验。
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本文引用的文献
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