一种在不到3小时内水解并测定皮摩尔量蛋白质氨基酸组成的方法。
A method for hydrolyzing and determining the amino acid compositions of picomole quantities of proteins in less than 3 hours.
作者信息
Lookhart G L, Jones B L, Cooper D B, Hall S B
出版信息
J Biochem Biophys Methods. 1982 Dec;7(1):15-23. doi: 10.1016/0165-022x(82)90032-x.
A method is described for quantitatively hydrolyzing proteins in 45 min and for analyzing the hydrolysates by high-performance liquid chromatography in an additional 52 min. The alpha-amino acids were detected by the fluorescence of their omicron-phthaldialdehyde derivatives. Ten picomoles of each of the commonly occurring alpha-amino acids could be reliably determined. The method described yielded OPA-ethanethiolamino acid derivatives that were stable for 1 1/2 h and the HPLC method produced a better separation than previously published methods.
本文描述了一种方法，可在45分钟内对蛋白质进行定量水解，并在额外的52分钟内通过高效液相色谱法对水解产物进行分析。α-氨基酸通过其邻苯二甲醛衍生物的荧光进行检测。十种常见α-氨基酸中的每一种的10皮摩尔都能被可靠测定。所述方法产生的OPA-乙硫醇氨基酸衍生物可稳定1.5小时，并且该高效液相色谱法比以前发表的方法具有更好的分离效果。
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