Goldfine I D, Jones A L, Hradek G T, Wong K Y, Mooney J S
Science. 1978 Nov 17;202(4369):760-3. doi: 10.1126/science.715440.
Electron microscope autoradiographs were prepared of IM-9 human cultured lymphocytes incubated with iodine-125-labeled insulin. With the use of [125I]insulin and Ilford L-4 emulsion, the technique had a resolution half-distance of approximately 0.085 micrometer. Autoradiographs revealed a time-dependent entry of insulin into the cell interior that was maximal after 30 minutes of incubation. At this time point nearly 40 percent of the [125I]insulin was in the interior of the cell at a distance 1 micrometer or greater from the plasma membrane. Grain distribution and volume density analyses revealed that the intracellular insulin was concentrated in the endoplasmic reticulum and nuclear membrane.
制备了用碘 - 125标记的胰岛素孵育的IM - 9人培养淋巴细胞的电子显微镜放射自显影片。使用[125I]胰岛素和伊尔福L - 4乳剂，该技术的分辨率半距离约为0.085微米。放射自显影片显示胰岛素进入细胞内部呈现时间依赖性，孵育30分钟后达到最大值。在这个时间点，近40%的[125I]胰岛素位于距离质膜1微米或更远的细胞内部。颗粒分布和体积密度分析表明，细胞内的胰岛素集中在内质网和核膜中。
