Bowden G H, Hardie J M, Fillery E D
J Dent Res. 1976 Jan;55:A192-204. doi: 10.1177/002203457605500112011.
Actinomyces have a complex antigenic structure that includes neutral cell wall carbohydrate and some polypeptide containing charged antigens. Wall carbohydrate can be prepared in a relatively pure form from cell walls or by DEAE Sephadex chromatography of acid extracts and supernatant culture fluid. Charged antigens are generally eluted in groups by batch-elution from columns. Cell wall carbohydrate can be responsible for species-specific reactions and cross-reactions, and strains may possess more than one cell wall carbohydrate determinant. The charged antigens also can be species specific, but they cause some cross-reactions, particularly those between the A israelii serotypes. Autoclave extracts of strains, tested against antiserums reacting with cell wall carbohydrate, may be valuable in routine identification of isolates.
放线菌具有复杂的抗原结构，包括中性细胞壁碳水化合物和一些含有带电荷抗原的多肽。细胞壁碳水化合物可以从细胞壁以相对纯的形式制备，或者通过对酸性提取物和上清液培养液进行二乙氨基乙基葡聚糖（DEAE Sephadex）色谱法来制备。带电荷的抗原通常通过柱上分批洗脱成组洗脱。细胞壁碳水化合物可导致种特异性反应和交叉反应，并且菌株可能具有不止一种细胞壁碳水化合物决定簇。带电荷的抗原也可以是种特异性的，但它们会引起一些交叉反应，特别是以色列放线菌血清型之间的交叉反应。针对与细胞壁碳水化合物反应的抗血清测试的菌株高压灭菌提取物，可能在分离株的常规鉴定中具有价值。
