Ferrando R, Truhaut R, Raynaud J P, Spanoghe J P
Toxicology. 1978 Oct;11(2):167-83. doi: 10.1016/s0300-483x(78)91039-9.
To check the possible toxicity risks of the meat from swine fed with a residue producing feed additive: Carbadox, a new methodology was used. It is the "Relay Toxicity". Swine were fed with a high dose of the additive and sacrificed without any withdrawal. In such conditions meat contains a high level of residues. The frozen meat was given daily--after thawing--at 100 g or 200 g/dog to 12 beagle dogs, 6 females and 6 males, sacrificed when 87.5 months old (85 months on experiment). On these animals, no anomaly was found: on weight gain and health; on fertility and reproductive performances; on hematology and biochemical values of blood and urine; and after careful macroscopic or microscopic examinations of the animals at safrice. We were able to obtain a safety factor above 9000 when Carbadox is used at the maximum level approved 50 ppm, and the withdrawal 4 weeks before slaughtering. To conclude, the absence of anomaly allows us to confirm the safety for the human consumer when Carbadox is used as a feed additive for swine.
为检测用产残留饲料添加剂卡巴氧喂养的猪的肉可能存在的毒性风险，采用了一种新方法，即“接力毒性”法。猪被投喂高剂量的该添加剂且未经过任何停药期就被宰杀。在这种情况下，猪肉含有高水平的残留物。将冷冻肉解冻后，每天以100克或200克/只的量喂给12只比格犬（6只雌性和6只雄性），这些犬在87.5月龄（实验进行85个月）时被宰杀。在这些动物身上，未发现任何异常：体重增加和健康状况方面；生育能力和繁殖性能方面；血液和尿液的血液学及生化指标方面；以及在宰杀时对动物进行仔细的宏观或微观检查后均未发现异常。当卡巴氧以批准的最大水平50 ppm使用且在屠宰前停药4周时，我们能够获得超过9000的安全系数。总之，未发现异常使我们能够确认，当卡巴氧用作猪的饲料添加剂时，对人类消费者是安全的。
