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特发性高脂蛋白血症中极低密度脂蛋白(VLDL)中的载脂蛋白C

Apolipoproteins C in very low density lipoproteins (VLDL) in idiopathic hyperlipoproteinaemia.

作者信息

Kłosiewicz-Latoszek L, Naruszewicz M, Szostak W B, Nowicka G

出版信息

Acta Physiol Pol. 1982 Jan-Apr;33(1-2):57-63.

PMID:7158381
Abstract

The purpose of this study was an assessment of the relative proportions of apolipoprotein C subfractions in the VLDL fraction, which could contribute to explaining the development of metabolic disturbances in hyper-pre-beta-lipoproteinaemias. The study was carried out in 21 patients with hyperlipoproteinaemia type IIa, IIb, III, IV and in 8 subjects without lipid metabolism disturbances. Apolipoproteins C were separated in disc electrophoresis on polyacrylamide gel (PAGE) with added 8-M of urea. After the electrophoresis three main subfractions were obtained and were identified according to their electrophoretic mobility as Apo CII, Apo CIII1 and Apo CIII2. The relative per cent proportions of apolipoproteins C were determined by densitometry. A correlation was found between triglyceride concentration in the VLDL fraction and the relative amounts of Apo CII and Apo CIII1. Moreover, a raised ratio of the lipoprotein lipase inhibitor (Apo CIII1) to the activator of this enzyme (Apo CII) was demonstrated in hyperlipoproteinaemias type IIb, III and IV, which may suggest decreased susceptibility of these lipoproteins to the action of lipoprotein lipase.

摘要

本研究的目的是评估极低密度脂蛋白(VLDL)组分中载脂蛋白C亚组分的相对比例,这可能有助于解释高前β脂蛋白血症中代谢紊乱的发生发展。该研究在21例IIa型、IIb型、III型、IV型高脂血症患者以及8名无脂质代谢紊乱的受试者中进行。载脂蛋白C在添加了8 M尿素的聚丙烯酰胺凝胶圆盘电泳(PAGE)中分离。电泳后获得了三个主要亚组分,并根据其电泳迁移率鉴定为载脂蛋白CII、载脂蛋白CIII1和载脂蛋白CIII2。通过光密度测定法确定载脂蛋白C的相对百分比。发现VLDL组分中的甘油三酯浓度与载脂蛋白CII和载脂蛋白CIII1的相对含量之间存在相关性。此外,在IIb型、III型和IV型高脂血症中,脂蛋白脂肪酶抑制剂(载脂蛋白CIII1)与该酶激活剂(载脂蛋白CII)的比例升高,这可能表明这些脂蛋白对脂蛋白脂肪酶作用的敏感性降低。

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