酒精对加州海兔神经元起搏活动的影响。
Effects of alcohols upon pacemaker activity in neurons of Aplysia californica.
作者信息
Silver L H, Treistman S N
出版信息
Cell Mol Neurobiol. 1982 Sep;2(3):215-26. doi: 10.1007/BF00711149.
Abstract
- Alcohols have been used as pharmacological tools to probe the nature of action-potential gates and channels. In this study, we examine the effects of alcohols upon activity patterns in Aplysia neurons. 2. Ethanol at concentrations of 0.4-0.6 M induces bursting pacemaker activity (BPA) in previously silent cells. The same effect is produced with 40-60 mM concentrations of butanol, suggesting that this induction is not due to osmotic effects. 3. Voltage-clamp measurements indicate that the induction of BPA is accompanied by the appearance of a negative-slope resistance (NSR) region in the steady-state current-voltage relationship of the cell. The induction of BPA and a NSR region in silent cells is antagonized by lowered temperatures. 4. Ethanol concentrations which produce BPA and a NSR region in silent cells abolish both of these normally present characteristics in endogenous bursters. This suggests that whatever membrane components are moved into optimal configuration for the expression of BPA in silent cells are shifted out of optimal configuration in endogenous bursters, by similar ethanol concentrations.
摘要
- 酒精一直被用作药理学工具来探究动作电位门控和通道的性质。在本研究中，我们研究了酒精对海兔神经元活动模式的影响。2. 浓度为0.4 - 0.6 M的乙醇可在先前静止的细胞中诱导爆发性起搏活动（BPA）。40 - 60 mM浓度的丁醇也会产生相同的效果，这表明这种诱导并非由于渗透作用。3. 电压钳测量表明，BPA的诱导伴随着细胞稳态电流 - 电压关系中负斜率电阻（NSR）区域的出现。低温可拮抗静止细胞中BPA和NSR区域的诱导。4. 在静止细胞中产生BPA和NSR区域的乙醇浓度会消除内源性爆发性细胞中这两种正常存在的特征。这表明，在静止细胞中，无论何种膜成分被移动到用于表达BPA的最佳构型，在类似乙醇浓度下，内源性爆发性细胞中的这些膜成分都会从最佳构型中移出。
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