Anderson D C, Wible L J, Hughes B J, Smith C W, Brinkley B R
Cell. 1982 Dec;31(3 Pt 2):719-29. doi: 10.1016/0092-8674(82)90326-9.
Indirect immunofluorescent (IIF) techniques employing antitutulin and anticentrosome antibodies were modified for studies of migrating polymorphonuclear (PMNs) leukocytes exposed to gradients or uniform concentrations of chemotactic factor (CF). No significant changes in microtubule (MT) number per cell occurred with chemotactic activation. Significant increases in average MT length per cell occurred upon exposure to gradients or uniform concentrations of CFs. Elongation of MT parallel to the direction of cell migration occurred coincident with shortening of other sets of MTs perpendicular to the direction of cell migration. MT extended radially from a single microtubule organizing center (MTOC) in greater than 99.9% of cells. Following chemotactic activation, the location of the MTOC was observed between nuclear lobes in 65% of fully polarized cells, "posterior" to the nucleus in congruent to 34% and "anterior" in less than 1%. Thus alterations of MT array occur coincident with cell orientation or migration in response to chemotactic stimuli.
采用抗微管蛋白和抗中心体抗体的间接免疫荧光（IIF）技术经过改良，用于研究暴露于趋化因子（CF）梯度或均匀浓度下的迁移多形核（PMN）白细胞。趋化激活后，每个细胞的微管（MT）数量没有显著变化。暴露于CF梯度或均匀浓度后，每个细胞的平均MT长度显著增加。与细胞迁移方向平行的MT伸长与垂直于细胞迁移方向的其他MT缩短同时发生。在超过99.9%的细胞中，MT从单个微管组织中心（MTOC）径向延伸。趋化激活后，在65%的完全极化细胞中，MTOC位于核叶之间，在约34%的细胞中位于核“后方”，在不到1%的细胞中位于核“前方”。因此，MT阵列的改变与细胞对趋化刺激的定向或迁移同时发生。
