Unsicker K, Millar T J, Hofmann H D
Dev Neurosci. 1982;5(5-6):412-7. doi: 10.1159/000112701.
Rat adrenal medullary cells, taken at different postnatal stages (1, 4, 10 and 14 days), were cultured in serum-containing medium with and without nerve growth factor (NGF 2.5 S, 50 ng/ml) to investigate their dependence on NGF for survival and maintenance of extended neurites. It was found that medullary cells depend on NGF for survival at day 1 and, to a lesser extent, at day 4. Neurite-like processes extended in response to NGF treatment breakdown when NGF is withdrawn. NGF-treated adrenal medullary cells displayed a pronounced tendency to form aggregates. It was also shown that plating adrenal medullary cells at higher cell densities, which largely increased the numbers of fibroblast-like cells present, may substitute for NGF with respect to survival.
取不同出生后阶段（1天、4天、10天和14天）的大鼠肾上腺髓质细胞，在含有和不含有神经生长因子（NGF 2.5 S，50 ng/ml）的含血清培养基中培养，以研究它们对NGF的生存依赖性以及延长神经突的维持情况。结果发现，髓质细胞在第1天依赖NGF生存，在第4天对NGF的依赖程度较低。当撤去NGF时，因NGF处理而延伸出的类神经突会断裂。经NGF处理的肾上腺髓质细胞表现出明显的聚集倾向。还表明，以较高细胞密度接种肾上腺髓质细胞，这在很大程度上增加了成纤维细胞样细胞的数量，就生存而言可能替代NGF。
