Mirell C J, Bekhor I
FEBS Lett. 1982 Dec 13;150(1):117-21. doi: 10.1016/0014-5793(82)81316-1.
Oviduct chromatin was isolated from both estrogenized and non-estrogenized hens. Extraction of the chromatin with 2 M NaCl removed a majority of the proteins, and the resulting DNA was then separated into two components: (1) a major fraction which was virtually protein-free; and (2) a minor fraction which was complexed with proteins. It was found that the DNA fraction that is complexed with proteins contained ovalbumin gene sequences and that the concentration of these sequences could be boosted by estrogen-treatment.
从经雌激素处理和未经雌激素处理的母鸡中分离出输卵管染色质。用2M氯化钠提取染色质可去除大部分蛋白质，然后将所得的DNA分离成两个组分：（1）几乎不含蛋白质的主要部分；（2）与蛋白质复合的次要部分。发现与蛋白质复合的DNA部分含有卵清蛋白基因序列，并且这些序列的浓度可通过雌激素处理而提高。
