Baurmann H, Chioralia G
Graefes Arch Clin Exp Ophthalmol. 1982;219(6):298-300. doi: 10.1007/BF00231419.
In addition to previous fluorescence-microscopic investigations on ocular barriers, quantitative fluorescence-photometric measurements on the eye tissues of albinotic rats were performed. The eyes were enucleated 5-30 min after intravenous injection, and tissue samples were compared with analogous eye tissues of untreated animals. The results were (I) different increases of measured data in the region of endothelium and stroma of the cornea, of vessels and stroma of the ciliary body and iris, of choroid and choriocapillaris, as well as of pigment epithelium and retina vessels; (II) no increase of the photometer values was observed in the epithelium of the cornea and in all other external and internal layers of the retina. It is to be assumed that in contrast to the tissues and vessels mentioned in (I), no measurable amounts of fluorescein have penetrated into the retina layers mentioned in (II). Although the fluorescence-photometric method may not be absolutely conclusive, it could be established that measurements are more exact than the qualitative method performed by fluorescence-microscopic observation.
除了之前对眼部屏障进行的荧光显微镜研究外，还对白化病大鼠的眼组织进行了定量荧光光度测量。在静脉注射后5 - 30分钟摘除眼球，将组织样本与未处理动物的类似眼组织进行比较。结果如下：（I）角膜内皮和基质区域、睫状体和虹膜的血管和基质、脉络膜和脉络膜毛细血管以及色素上皮和视网膜血管区域的测量数据有不同程度的增加；（II）在角膜上皮以及视网膜的所有其他外层和内层中未观察到光度计值的增加。可以假定，与（I）中提到的组织和血管相比，没有可测量量的荧光素渗透到（II）中提到的视网膜层。尽管荧光光度法可能并非绝对结论性的，但可以确定测量比通过荧光显微镜观察进行的定性方法更精确。
