Kern D H, Campbell M A, Cochran A J, Burk M W, Morton D L
Int J Cancer. 1982 Dec 15;30(6):725-9. doi: 10.1002/ijc.2910300608.
Of 1,014 human solid tumors of various histologic types, 690 (68%) showed evidence of colony formation within 2 to 4 weeks. Tumors which grew particularly well were colon carcinoma (104/175), melanoma (134/155), lung carcinoma (62/85), breast cancer (100/140), ovarian carcinoma (50/67), and sarcoma (72/122). Histologic examination indicated that the colony-forming cells retained functional and morphologic features similar to those of the original tumor. Plating efficiencies varied between 0.01% and 0.2%, and the numbers of colonies observed formed a direct linear correlation with the number of cells plated. Recovery of viable tumor cells was increased when enzymatic tumor dissociation was used rather than a mechanical method. A simplified, supplemented medium resulted in improved cloning efficiencies when compared to previously reported methods (Hamburger and Salmon, 1977 b).
在1014例各种组织学类型的人类实体瘤中，690例（68%）在2至4周内显示出集落形成的证据。生长特别良好的肿瘤有结肠癌（104/175）、黑色素瘤（134/155）、肺癌（62/85）、乳腺癌（100/140）、卵巢癌（50/67）和肉瘤（72/122）。组织学检查表明，集落形成细胞保留了与原始肿瘤相似的功能和形态特征。接种效率在0.01%至0.2%之间，观察到的集落数量与接种的细胞数量呈直接线性相关。与机械方法相比，采用酶解肿瘤的方法可提高存活肿瘤细胞的回收率。与先前报道的方法（Hamburger和Salmon，1977b）相比，一种简化的补充培养基可提高克隆效率。
