Verheijen R H, Feitz W F, Kenemans P, Vooys G P, Herman C J
Br J Cancer. 1985 Nov;52(5):707-12. doi: 10.1038/bjc.1985.247.
Single time point assessment is usually employed in the Human Tumour Cloning System as the only parameter for in vitro growth. This does not seem to give a fair expression of the dynamic biological properties of tumour growth and time dependent effects, e.g. of cytotoxic drugs. We studied the time course of colony formation in temporal growth patterns (TGPs) and compared this method of growth evaluation with conventional single time point assessment in 57 samples of ovarian tumour cultures in the HTCS. A first advantage of the use of TGPs is that more cultures become evaluable, as this assessment over time can detect a rise in the number of colonies in dishes where colony-like clumps have initially been seeded. Thus only 28 of the cultures were evaluable for single time point assessment, whereas 57 were available for TGP evaluation. Growth was more often seen at TGP evaluation (14/57) than at single day assessment (8/57). Evaluation of growth over the course of time potentially allows detection of sensitivity to drugs. Furthermore TGPs reflect the dynamics of biological growth. These features cannot be studied in single time point assessment.
在人类肿瘤克隆系统中，通常采用单次时间点评估作为体外生长的唯一参数。这似乎无法公平地体现肿瘤生长的动态生物学特性以及时间依赖性效应，例如细胞毒性药物的效应。我们研究了时间生长模式（TGPs）中集落形成的时间进程，并将这种生长评估方法与传统的单次时间点评估方法在人类肿瘤克隆系统（HTCS）中的57个卵巢肿瘤培养样本中进行了比较。使用TGPs的第一个优势在于更多的培养物变得可评估，因为随着时间的推移进行这种评估能够检测到最初接种了集落样团块的培养皿中集落数量的增加。因此，只有28个培养物可用于单次时间点评估，而有57个可用于TGP评估。在TGP评估时观察到生长的情况（14/57）比在单日评估时（8/57）更为常见。对一段时间内生长情况的评估有可能检测出对药物的敏感性。此外，TGPs反映了生物生长的动态过程。这些特性在单次时间点评估中无法进行研究。
