Salvadori C, Farinotti R, Dauphin A, Mahuzier G
Ann Biol Clin (Paris). 1982;40(6):667-72.
We propose a method to quantify quinine in biological fluids using liquid chromatography. After alkalinisation, samples are extracted with a chloroform-isoamyl alcohol mixture; in the case of urinary samples, a preliminary enzymatic hydrolysis is performed. Extracts are analyzed by adsorption chromatography. A mobile phase composed of methanol-acetonitril-ammonia in used to elute. A post-column reaction with sulfuric acid allows a fluorimetric detection. With this method concentration as low as 0.05 mg/l can be measured. Variation coefficient is about 2.5 p. cent Quinine, its metabolites and dihydroquinine are well separated; thus it is a very useful method for pharmacokinetic and metabolic studies.
我们提出了一种使用液相色谱法定量生物流体中奎宁的方法。碱化后，样品用氯仿 - 异戊醇混合物萃取；对于尿液样品，需进行初步酶水解。提取物通过吸附色谱法进行分析。使用由甲醇 - 乙腈 - 氨组成的流动相进行洗脱。与硫酸的柱后反应实现荧光检测。用这种方法可测量低至0.05mg/l的浓度。变异系数约为2.5%。奎宁、其代谢物和二氢奎宁能得到很好的分离；因此它是药代动力学和代谢研究中非常有用的方法。
