纯化肺炎球菌溶血素的特性
Properties of purified pneumococcal hemolysin.
作者信息
Johnson M K
出版信息
Infect Immun. 1972 Nov;6(5):755-60. doi: 10.1128/iai.6.5.755-760.1972.
Abstract
With a purified preparation of pneumococcal hemolysin, a sigmoid relationship was found to exist between lysin concentration and hemolytic activity. Hemolysis was inhibited by a high ratio of erythrocytes to lysin, suggesting a multi-hit mechanism of action. Reaction rate decreased rapidly with time, possibly due to competition between ghosts and unlysed erythrocytes for fixation of lysin. The effects of pH and various agents on the processes of lysin adsorption and hemoglobin release were determined. The pneumococcal preparation did not possess nicotinamide adenine dinucleotide glycohydrolase activity.
摘要
使用纯化的肺炎球菌溶血素制剂，发现溶素浓度与溶血活性之间存在S形关系。高比例的红细胞与溶素可抑制溶血，提示存在多重打击作用机制。反应速率随时间迅速降低，这可能是由于空壳红细胞与未裂解红细胞竞争溶素固定所致。测定了pH值和各种试剂对溶素吸附和血红蛋白释放过程的影响。肺炎球菌制剂不具有烟酰胺腺嘌呤二核苷酸糖水解酶活性。
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