Brat S V, Williams G M
Cancer Lett. 1982 Nov-Dec;17(2):213-6. doi: 10.1016/0304-3835(82)90034-9.
To study the possibility that liver metabolism of acrylonitrile (ACN) could produce a stable carcinogenic metabolite that causes the brain tumors produced by ACN, the ability of hepatocytes to generate a stable transportable mutagenic metabolite in culture was studied. ACN did not produce sister chromatid exchanges (SCEs) in Chinese hamster ovary (CHO) cells, but a significant increase was produced when CHO cells were co-cultured with freshly isolated rat hepatocytes. Induction of SCE in CHO cells co-cultured with hepatocytes suggests that the liver cells metabolize ACN and the reactive metabolite is transported from them to the CHO cells.
为了研究丙烯腈（ACN）的肝脏代谢是否会产生一种稳定的致癌代谢物从而导致ACN诱发脑肿瘤，研究了肝细胞在培养中产生稳定的可转运诱变代谢物的能力。ACN在中国仓鼠卵巢（CHO）细胞中不会产生姐妹染色单体交换（SCE），但当CHO细胞与新鲜分离的大鼠肝细胞共培养时，会产生显著增加。与肝细胞共培养的CHO细胞中SCE的诱导表明，肝细胞代谢ACN，并且活性代谢物从肝细胞转运到CHO细胞中。
