Forkert P G, Custer E M, Alpert A J, Ansari G A, Reynolds E S
Exp Lung Res. 1982 Dec;4(1):67-77. doi: 10.3109/01902148209039250.
Lactate dehydrogenase (LD) activity and its isozyme profile in mouse lung homogenate was affected by oral administration of 1,1-dichloroethylene (1,1-DCE). Following 100 mg 1,1-DCE/kg, LD-3 increased significantly. After 200 mg 1,1-DCE/kg, LD-5 increased whereas LD-1 and LD-2 decreased, with a resultant higher M:H ratio than controls. In contrast, elevated LD activity in serum following 1,1-DCE was predominantly associated with striking increases in total activity and changes in isozyme patterns resulting in a decrease in the M:H ratio. LD activity in liver and erythrocytes were unaffected by 1,1,-DCE administration. Although total activity in kidney was decreased, no changes were detected in the isozyme profile. Pulmonary damage induced by 1,1-DCE was reflected in significant increases in total activity and all isozymes in bronchopulmonary lavage fluids. Thus, detection of lung-derived LD activity in lung lavage fluids can be a useful index of pulmonary airway injury.
口服1,1 - 二氯乙烯（1,1 - DCE）会影响小鼠肺匀浆中乳酸脱氢酶（LD）的活性及其同工酶谱。给予100 mg 1,1 - DCE/kg后，LD - 3显著增加。给予200 mg 1,1 - DCE/kg后，LD - 5增加，而LD - 1和LD - 2减少，导致M:H比值高于对照组。相比之下，1,1 - DCE处理后血清中LD活性升高主要与总活性显著增加以及同工酶模式变化有关，导致M:H比值降低。肝脏和红细胞中的LD活性不受1,1 - DCE给药的影响。虽然肾脏中的总活性降低，但同工酶谱未检测到变化。1,1 - DCE诱导的肺损伤表现为支气管肺泡灌洗液中总活性和所有同工酶显著增加。因此，检测肺灌洗液中肺源性LD活性可作为肺气道损伤的有用指标。
