Ashorn R
Acta Pathol Microbiol Immunol Scand C. 1982 Dec;90(6):339-45. doi: 10.1111/j.1699-0463.1982.tb01460.x.
The effect of porcine spleen dialysate was studied on delayed-type hypersensitivity reactions, as well as on the phagocytic capacity and intracellular prostaglandin levels of peritoneal macrophages. Porcine spleen cell dialysate was fractionated on Sephadex G-10 and these fractions, as well as unfractionated dialysate, were injected intraperitoneally into antigen-primed guinea pigs. The guinea pigs were tested for delayed-type hypersensitivity towards PPD and SK-SD. The results show that unfractionated spleen cell dialysate augmented slightly the delayed-type hypersensitivity reactions, and after fractionation a significant augmentory activity was detected in two adjacent, early eluting fractions. This active elution region corresponds to the elution region where most of the guinea pig DTH augmenting activity of leukocyte dialysate is also detected. Furthermore, these fractions significantly augmented the phagocytic capacity of peritoneal macrophages, and decreased the intracellular levels of prostaglandin PGE2, thromboxan B2 and prostacyclin. These results indicate that porcine spleen cells from non-immunized animals can be used as a source for a non-specifically acting immunomodulatory preparation, which resembles that found in human leukocyte dialysate. These immunomodulatory substance(s) seem to operate via monocyte activation.
研究了猪脾透析液对迟发型超敏反应以及对腹腔巨噬细胞吞噬能力和细胞内前列腺素水平的影响。将猪脾细胞透析液在葡聚糖G - 10上进行分级分离，然后将这些级分以及未分级的透析液腹腔注射到用抗原致敏的豚鼠体内。检测豚鼠对结核菌素纯蛋白衍生物（PPD）和链激酶 - 链道酶（SK - SD）的迟发型超敏反应。结果表明，未分级的脾细胞透析液使迟发型超敏反应略有增强，分级分离后在两个相邻的早期洗脱级分中检测到显著的增强活性。这个活性洗脱区域与在白细胞透析液中检测到的大部分豚鼠迟发型超敏反应增强活性的洗脱区域相对应。此外，这些级分显著增强了腹腔巨噬细胞的吞噬能力，并降低了细胞内前列腺素PGE2、血栓素B2和前列环素的水平。这些结果表明，来自未免疫动物的猪脾细胞可用作一种非特异性免疫调节制剂的来源，这种制剂类似于在人白细胞透析液中发现的制剂。这些免疫调节物质似乎通过单核细胞激活起作用。
