Karhumäki E
Department of Biomedical Sciences, University of Tampere, Finland.
Acta Pathol Microbiol Immunol Scand C. 1987 Dec;95(6):251-6. doi: 10.1111/j.1699-0463.1987.tb00038.x.
The effects of human leukocyte, porcine spleen and bovine liver dialysate fractions on Listeria resistance were measured by survival studies and by assessing the capacity of peritoneal macrophages to produce superoxide anion (0-2) and prostaglandins. Leukocyte (DLE) and other tissue dialysates were fractionated on a Sephadex G-10 column. Thereafter the significant activities were found in fraction III of DLE, fraction II of porcine spleen and fraction II + III of bovine liver dialysate. The treatment with active porcine spleen dialysate fraction increased the capacity of peritoneal macrophages to generate superoxide anion. On the other hand, this fraction significantly decreased the production of prostaglandin PGE2 and thromboxan B2. These results may indicate that all dialysates can be a source of a non-specifically-acting immunomodulatory preparation and that the infection-resistance-increasing substances seem to operate via the monocyte/macrophage activation.
通过生存研究以及评估腹膜巨噬细胞产生超氧阴离子（O₂⁻）和前列腺素的能力，来测定人白细胞、猪脾脏和牛肝脏透析液组分对李斯特菌抵抗力的影响。白细胞（DLE）和其他组织透析液在葡聚糖G - 10柱上进行分级分离。此后，在DLE的组分III、猪脾脏的组分II以及牛肝脏透析液的组分II + III中发现了显著活性。用活性猪脾脏透析液组分处理可提高腹膜巨噬细胞产生超氧阴离子的能力。另一方面，该组分显著降低了前列腺素PGE₂和血栓素B₂的产生。这些结果可能表明，所有透析液都可能是非特异性免疫调节制剂的来源，并且增加抗感染能力的物质似乎是通过单核细胞/巨噬细胞激活发挥作用的。
