Karhumäki E, Ashorn R, Krohn K
Acta Pathol Microbiol Immunol Scand C. 1985 Jun;93(3):111-6. doi: 10.1111/j.1699-0463.1985.tb02931.x.
The effect of dialyzable transfer factor (TFd) on Listeria resistance was measured by survival studies and by assessing phagocytic capacity of peritoneal macrophages. Unfractionated dialysates from human leukocytes (DLE), bovine liver, porcine spleen and kidney as well as saline were injected i.p. into NMRI mice 72 h before the i.p. injection of 1-3 x 10(6) Listeria organisms. The results show that DLE, porcine spleen and bovine liver dialysate increased the LD50 5-20 times. Porcine kidney dialysate had no effect on the survival of the mice. After the fractionation of porcine spleen dialysate on Sephadex G-10 column, a significant activity was found in two fractions, II and IX. When active fractions were given together (II + IX) i.p. three days prior to the infection with listeria organisms, the survival of mice increased significantly, whereas no effect was seen when the fractions were given i.v. and the bacteria i.p. Also the treatment with active fractions increased significantly the phagocytic capacity of peritoneal macrophages. Taken together, our results suggest that the Listeria protective substances seem to operate via monocyte activation.
通过存活研究以及评估腹膜巨噬细胞的吞噬能力，测定了可透析转移因子（TFd）对李斯特菌抵抗力的影响。在经腹腔注射1 - 3×10⁶ 个李斯特菌菌体前72小时，将来自人白细胞的未分级透析液（DLE）、牛肝、猪脾和猪肾的透析液以及生理盐水经腹腔注射到NMRI小鼠体内。结果显示，DLE、猪脾和牛肝透析液使半数致死剂量（LD50）增加了5至20倍。猪肾透析液对小鼠的存活没有影响。在Sephadex G - 10柱上对猪脾透析液进行分级分离后，在组分II和IX中发现了显著活性。当在感染李斯特菌菌体前三天经腹腔一起给予活性组分（II + IX）时，小鼠的存活率显著提高，而当经静脉给予这些组分且经腹腔注射细菌时则未观察到效果。此外，用活性组分处理也显著提高了腹膜巨噬细胞的吞噬能力。综上所述，我们的结果表明，李斯特菌保护性物质似乎通过单核细胞激活发挥作用。
