Theoretical analysis of specimen cooling rate during impact freezing and liquid-jet freezing of freeze-etch specimens.
作者信息
Kopstad G, Elgsaeter A
出版信息
Biophys J. 1982 Nov;40(2):163-70. doi: 10.1016/S0006-3495(82)84471-8.
Abstract
We have carried out a theoretical analysis of specimen cooling rate under ideal conditions during impact freezing and liquid-jet freezing. The analysis shows that use of liquid helium instead of liquid nitrogen as cooling medium during impact freezing results in an increase in a specimen cooling rate of no more than 30-40%. We have further shown that when both impact freezing and liquid-jet freezing are conducted at liquid nitrogen temperature, the two methods give approximately the same specimen cooling rate under ideal conditions except for a thin outer layer of the specimen. In this region impact freezing yields the highest cooling rate.
摘要
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本文引用的文献
1
[FREEZE-FIXATION OF LIVING CELLS AND ITS USE IN ELECTRON MICROSCOPY].
Z Zellforsch Mikrosk Anat. 1964 Apr 28;62:546-80.
2
Theoretical analysis of the ice crystal size distribution in frozen aqueous specimens.
Biophys J. 1982 Nov;40(2):155-61. doi: 10.1016/S0006-3495(82)84470-6.
3
Freezing in a propane jet and its application in freeze-fracturing.
Mikroskopie. 1980 Sep;36(5-6):129-40.
4
Organization of acetylcholine receptors in quick-frozen, deep-etched, and rotary-replicated Torpedo postsynaptic membrane.
J Cell Biol. 1979 Jul;82(1):150-73. doi: 10.1083/jcb.82.1.150.
5
Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release.
J Cell Biol. 1979 May;81(2):275-300. doi: 10.1083/jcb.81.2.275.
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