Tsang A Y, Bentz R R, Schork M A, Sodeman T M
Am J Clin Pathol. 1978 Nov;70(5):816-20. doi: 10.1093/ajcp/70.5.816.
The effects of combined drugs were compared uith the effects of single drugs in vitro against Mycobacterium kansasii. The single drugs isoniazid 1.0 microgram/ml, streptomycin 2.0 microgram/ml, and ethambutol 5.0 microgram/ml and the combinations of 1.0 microgram/ml isoniazid and 2.0 microgram/ml streptomycin, 1.0 microgram/ml isoniazid and 5.0 ethambutal and 1.0 microgram/ml isoniazid, 2.0 microgram/ml streptomycin and 5.0 microgram/ml ethambutol were evaluated as to their effects on M. kansasii organisms from 22 patients with pulmonary mycobacterial disease. These drugs were incorporated into Middlebrook 7H11 medium. Colonial growth was then observed and assigned numerical values for statistical analysis. The results showed that isoniazid was not significantly better than ethambutol. Streptomycin, however, was significantly better than both isoniazid and ethambutol alone. The double combinations were better than the single drugs, with isoniazid--streptomycin being better than isoniazid--ethambutol. The combination isoniazid--ethambutol was not significantly better than streptomycin alone. However, the triple combination of isoniazid--streptomycin--ethambutol was markedly better than all single drugs, better than isoniazid--ethambutol, but not better than isoniazid--streptomycin. Combined-drug testing thus showed a pattern of susceptibility not demonstrated by single-drug testing.
在体外将联合用药的效果与单一药物对堪萨斯分枝杆菌的效果进行了比较。单一药物包括1.0微克/毫升异烟肼、2.0微克/毫升链霉素和5.0微克/毫升乙胺丁醇，以及1.0微克/毫升异烟肼与2.0微克/毫升链霉素的组合、1.0微克/毫升异烟肼与5.0微克/毫升乙胺丁醇的组合、1.0微克/毫升异烟肼、2.0微克/毫升链霉素与5.0微克/毫升乙胺丁醇的组合，对22例肺部分枝杆菌病患者的堪萨斯分枝杆菌菌株的效果进行了评估。这些药物被加入到Middlebrook 7H11培养基中。然后观察菌落生长情况并赋予数值以便进行统计分析。结果显示，异烟肼并不比乙胺丁醇显著更好。然而，链霉素比单独使用异烟肼和乙胺丁醇都显著更好。联合用药比单一药物更好，异烟肼 - 链霉素组合比异烟肼 - 乙胺丁醇组合更好。异烟肼 - 乙胺丁醇组合并不比单独使用链霉素显著更好。然而，异烟肼 - 链霉素 - 乙胺丁醇三联组合明显优于所有单一药物，优于异烟肼 - 乙胺丁醇组合，但不比异烟肼 - 链霉素组合更好。因此，联合药物测试显示出一种单一药物测试未显示的敏感性模式。
