Wakisaka Y, Kawamura Y, Yasuda Y, Koizumi K, Nishimoto Y
J Antibiot (Tokyo). 1982 Jul;35(7):822-36. doi: 10.7164/antibiotics.35.822.
A selective medium containing 25 to 50 micrograms per ml of tunicamycin was devised to isolate micromonosporae from soil samples, making possible simple, preferential isolation of a variety of Micromonospora. When a large amount of Gram-negative bacteria was present in a sample, alkaline treatment (0.01 N NaOH, 5 approximately 10 minutes at 15 degrees C) wa employed to reduce the numbers. Using the tunicamycin agar medium, 1,585 strains of presumably different micromonosporae were obtained from 400 soil samples collected from various regions around the world. In average, 4 different Micromonospora strains could be located from one soil sample. This tunicamycin method made possible a concentrated screening method for new antibiotics from Micromonospora.
设计了一种每毫升含25至50微克衣霉素的选择性培养基，用于从土壤样品中分离小单孢菌，从而能够简单、优先地分离出多种小单孢菌。当样品中存在大量革兰氏阴性菌时，采用碱性处理（0.01 N NaOH，15℃下处理5至10分钟）来减少其数量。使用衣霉素琼脂培养基，从世界各地采集的400份土壤样品中获得了1585株可能不同的小单孢菌。平均而言，从一份土壤样品中可分离出4种不同的小单孢菌菌株。这种衣霉素方法使得从小单孢菌中筛选新抗生素的浓缩筛选方法成为可能。
