[高纯度头孢西丁C的制备]

[Preparation of highly purified cephamycin C].

作者信息

Kunishima M, Yabuuchi M, Masuda M, Imazumi K, Hamano H

出版信息

Jpn J Antibiot. 1982 Jul;35(7):1699-700.

Abstract

Preparation of highly purified cephamycin C (CM-C) is described in this report. The purification of CM-C was carried out by Sephadex LH-20 chromatography of the N-BOC-CM-C developed with MeOH followed by removal of the protecting group. Partially purified CM-C was further refined by Sephadex LH-20 chromatography developed with MeOH and Diaion HP-20 SS chromatography developed with H2O. CM-C thus obtained showed the UV absorption at 265 nm [E1% 1cm 184.4 (H2O)] which is the strongest absorption ever appeared in the literatures.

摘要

本报告描述了高纯度头孢西丁C（CM-C）的制备方法。CM-C的纯化是通过用甲醇展开的N-BOC-CM-C的葡聚糖凝胶LH-20色谱法，然后去除保护基团来进行的。部分纯化的CM-C通过用甲醇展开的葡聚糖凝胶LH-20色谱法和用水展开的Diaion HP-20 SS色谱法进一步精制。由此获得的CM-C在265nm处显示出紫外吸收[E1% 1cm 184.4（水）]，这是文献中出现过的最强吸收。