用于硫酸雌二醇-3放射免疫测定的特异性抗血清。

Specific antisera for the radioimmunoassay of estradiol-3-sulfate.

作者信息

Tanaka T, Suguro N, Shiratori Y, Kubodera A

出版信息

J Steroid Biochem. 1985 Feb;22(2):285-8. doi: 10.1016/0022-4731(85)90126-8.

Abstract

Antisera were prepared against two types of estradiol-3-sulfate-bovine serum albumin (BSA) conjugates. The haptens were coupled to BSA through the C-6 position in the steroid molecule by the glutaraldehyde (A) or the carbodiimide method (B). In comparison the antiserum produced by method A had a high affinity for estradiol-3-sulfate (Ka = 5.64 X 10(8) M-1); that produced by method B had an even higher affinity (Ka = 2.62 X 10(9) M-1). Furthermore the latter had no significant cross-reaction with other estrogen sulfates (less than 3.83%), and no cross-reaction with other steroids (less than 0.03%). The former revealed a little cross-reactivity with some of related steroids.

摘要

制备了针对两种硫酸雌二醇 -3- 牛血清白蛋白（BSA）偶联物的抗血清。半抗原通过戊二醛（方法A）或碳二亚胺法（方法B）在类固醇分子的C-6位与BSA偶联。相比之下，方法A产生的抗血清对硫酸雌二醇 -3- 具有高亲和力（Ka = 5.64×10⁸ M⁻¹）；方法B产生的抗血清具有更高的亲和力（Ka = 2.62×10⁹ M⁻¹）。此外，后者与其他雌激素硫酸盐无明显交叉反应（小于3.83%），与其他类固醇无交叉反应（小于0.03%）。前者与一些相关类固醇有轻微交叉反应。