人类膀胱癌细胞癌基因T24正常等位基因定位于11号染色体。

Localization of the normal allele of T24 human bladder carcinoma oncogene to chromosome 11.

作者信息

McBride O W, Swan D C, Santos E, Barbacid M, Tronick S R, Aaronson S A

出版信息

Nature. 1982 Dec 23;300(5894):773-4. doi: 10.1038/300773a0.

Abstract

The development of DNA-mediated gene transfer techniques has made it possible to identify transforming genes present in certain human tumour cells. Such genes have been shown to induce morphological transformation when used to transfect suitable assay cells. Recently a transforming gene has been isolated by molecular cloning techniques from the T24 (ref. 11) and EJ (ref. 12) human bladder carcinoma cell lines. This bladder carcinoma oncogene has been shown to be of human origin, less than six kilobase pairs (kbp) in size, and closely related to the onc genes (v-bas and v-ras) of BALB and Harvey murine sarcoma viruses. These transforming retroviruses arose in nature by transduction of cellular genes from mouse and rat cells, respectively. To understand better the relationship of the T24 oncogene with other human cellular genes, we have determined the chromosomal location of its normal allele within the human genome. We show here that it is carried on chromosome 11 in normal cells.

摘要

DNA介导的基因转移技术的发展使得鉴定某些人类肿瘤细胞中存在的转化基因成为可能。当用于转染合适的分析细胞时，这类基因已被证明能诱导形态转化。最近，通过分子克隆技术从T24（参考文献11）和EJ（参考文献12）人膀胱癌细胞系中分离出了一个转化基因。这个膀胱癌癌基因已被证明是人类起源的，大小小于6千碱基对（kbp），并且与BALB和哈维鼠肉瘤病毒的癌基因（v-bas和v-ras）密切相关。这些转化逆转录病毒分别是通过转导来自小鼠和大鼠细胞的细胞基因在自然界中产生的。为了更好地理解T24癌基因与其他人类细胞基因的关系，我们已经确定了其正常等位基因在人类基因组中的染色体定位。我们在此表明，它在正常细胞中位于11号染色体上。