McBride O W, Swan D C, Tronick S R, Gol R, Klimanis D, Moore D E, Aaronson S A
Nucleic Acids Res. 1983 Dec 10;11(23):8221-36. doi: 10.1093/nar/11.23.8221.
The identification of transforming genes in human tumor cells has been made possible by DNA mediated gene transfer techniques. To date, it has been possible to show that most of these transforming genes are activated cellular analogues of the ras oncogene family. To better understand the relationship between these oncogenes and other human genes, we have determined their chromosomal localization by analyzing human rodent somatic cell hybrids with molecularly cloned human proto-oncogene probes. It was possible to assign N-ras to chromosome 1 and regionally localize c-K-ras-1 and c-K-ras-2 to human chromosomes 6pter-q13 and 12q, respectively. These results along with previous studies demonstrate the highly dispersed nature of ras genes in the human genome. Previous reports indicated that the c-myb gene also resides on chromosome 6. It has been possible to sublocalize c-myb to the long arm of chromosome 6 (q15-q21). The non-random aberrations in chromosomes 1, 6 and 12 that occur in certain human tumors suggest possible etiologic involvement of ras and/or myb oncogenes in such tumors.
DNA介导的基因转移技术使得在人类肿瘤细胞中鉴定转化基因成为可能。迄今为止，已经能够表明这些转化基因中的大多数是ras癌基因家族的活化细胞类似物。为了更好地理解这些癌基因与其他人类基因之间的关系，我们通过用分子克隆的人类原癌基因探针分析人类-啮齿类体细胞杂种，确定了它们的染色体定位。已将N-ras定位于1号染色体，并将c-K-ras-1和c-K-ras-2分别区域定位于人类染色体6pter-q13和12q。这些结果与先前的研究一起证明了ras基因在人类基因组中的高度分散性质。先前的报道表明c-myb基因也位于6号染色体上。已将c-myb亚定位于6号染色体长臂（q15-q21）。在某些人类肿瘤中出现的1、6和12号染色体的非随机畸变提示ras和/或myb癌基因可能在此类肿瘤中参与病因学过程。
