Masserano J M, King C
Neuropharmacology. 1982 Nov;21(11):1163-7. doi: 10.1016/0028-3908(82)90174-5.
The loci coeruleus of freely moving cats were perfused bilaterally with acetylcholine at a dose of 0.001 microgram/microliter per min, while the animals were recorded polygraphically. The controls consisted of experiments in which no perfusions were done, and experiments in which the loci coeruleus were perfused bilaterally with saline, 1 microliter/min. The acetylcholine produced a sharp inhibition of rapid eye movement (REM) sleep, prolonged the sleep cycle and the REM interval and decreased the number of REM periods and the mean duration of the REM episodes. Total sleep time was increased. This was entirely due to a specific elevation by acetylcholine of deep slow-wave sleep; light slow-wave sleep was not affected. The data support the Hobson-McCarley hypothesis of how the brain controls the REM state, and also suggest that cholinergic stimulation of the locus coeruleus may be important for the shift from REM sleep into slow-wave sleep.
在自由活动的猫身上，以每分钟0.001微克/微升的剂量对双侧蓝斑进行乙酰胆碱灌注，同时用多导记录仪记录动物的情况。对照组包括未进行灌注的实验，以及以每分钟1微升的速度对双侧蓝斑进行生理盐水灌注的实验。乙酰胆碱对快速眼动（REM）睡眠产生了强烈抑制，延长了睡眠周期和REM间隔，减少了REM期的数量和REM发作的平均持续时间。总睡眠时间增加。这完全是由于乙酰胆碱特异性地提高了深度慢波睡眠；轻度慢波睡眠未受影响。这些数据支持了霍布森-麦卡利关于大脑如何控制REM状态的假说，也表明蓝斑的胆碱能刺激可能对从REM睡眠向慢波睡眠的转变很重要。
