Kuzmich S, Marky L A, Jones R A
Nucleic Acids Res. 1982 Oct 25;10(20):6265-71. doi: 10.1093/nar/10.20.6265.
The hexanucleotide d(CGTACG) has been synthesized by a phosphotriester method in which, for the first time, an O-6 protected deoxyguanosine derivative has been used to avoid side reactions of the guanine residues. Conformational analysis by circular dichroism shows that d(CGTACG) maintains a B form under conditions (5 M NaCl) where the all C/G hexanucleotide d(CG)3 adopts a Z form, even though d(CGTACG) is a fully alternating pyrimidine/purine molecular. The delta H for the helix-to-coil transition has been measured.
已通过磷酸三酯法合成了六核苷酸d(CGTACG),其中首次使用了O-6保护的脱氧鸟苷衍生物以避免鸟嘌呤残基的副反应。圆二色性的构象分析表明,尽管d(CGTACG)是完全交替的嘧啶/嘌呤分子,但在所有C/G六核苷酸d(CG)3呈Z型的条件下(5M NaCl),d(CGTACG)仍保持B型。已测量了螺旋-卷曲转变的ΔH。