嘌呤2-氨基对DNA构象和稳定性的影响。d[T(2-氨基A)]3的合成与构象分析。
The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3.
作者信息
Gaffney B L, Marky L A, Jones R A
出版信息
Nucleic Acids Res. 1982 Jul 24;10(14):4351-61. doi: 10.1093/nar/10.14.4351.
Abstract
A self-complementary hexanucleotide consisting of thymidine and 2-amino-deoxyadenosine, d(TA')3, has been synthesized by a solid phase phosphotriester method. Melting studies show that the additional hydrogen bond afforded by the 2-amino group substantially stabilizes the duplex. Moreover, conformational analysis using circular dichroism shows that a salt-induced conformational transition occurs, similar to the B leads to Z transition observed for d(CG)n oligonucleotides.
摘要
一种由胸苷和2-氨基脱氧腺苷组成的自互补六核苷酸d(TA')3，已通过固相磷酸三酯法合成。熔解研究表明，2-氨基提供的额外氢键极大地稳定了双链体。此外，使用圆二色性的构象分析表明，发生了盐诱导的构象转变，类似于在d(CG)n寡核苷酸中观察到的B型向Z型转变。
相似文献
1
The influence of the purine 2-amino group on DNA conformation and stability. Synthesis and conformational analysis of d[T(2-aminoA)]3.嘌呤2-氨基对DNA构象和稳定性的影响。d[T(2-氨基A)]3的合成与构象分析。
Nucleic Acids Res. 1982 Jul 24;10(14):4351-61. doi: 10.1093/nar/10.14.4351.
2
Synthesis and physical characterization of the self-complementary, alternating pyrimidine/purine hexanucleotide d[CGTACG].自我互补交替嘧啶/嘌呤六核苷酸d[CGTACG]的合成与物理表征
Nucleic Acids Res. 1982 Oct 25;10(20):6265-71. doi: 10.1093/nar/10.20.6265.
3
Specifically alkylated DNA fragments. Synthesis and physical characterization of d[CGC(O6Me)GCG] and d[CGT(O6Me)GCG].特定烷基化的DNA片段。d[CGC(O6Me)GCG]和d[CGT(O6Me)GCG]的合成及物理特性表征
Nucleic Acids Res. 1983 May 25;11(10):3393-403. doi: 10.1093/nar/11.10.3393.
4
Sequence specificity of Z-DNA formation in oligonucleotides.寡核苷酸中Z-DNA形成的序列特异性。
Biochem Int. 1988 Jul;17(1):121-31.
5
Spectroscopic studies of (m5dC-dG)3: thermal stability of B- and Z-forms.（m5dC-dG）3的光谱研究：B型和Z型的热稳定性
Nucleic Acids Res. 1983 Jul 11;11(13):4453-66. doi: 10.1093/nar/11.13.4453.
6
Solid phase synthesis of oligonucleotides on a crosslinked polyacrylmorpholide support.在交联聚丙烯酰吗啉载体上进行寡核苷酸的固相合成。
Nucleic Acids Symp Ser. 1980(7):233-41.
7
The left-handed Z-DNA conformation in oligodeoxynucleotides containing different amounts of AT base pairs: a far UV circular dichroism study.含不同数量AT碱基对的寡脱氧核苷酸中的左手Z-DNA构象：远紫外圆二色性研究
J Biomol Struct Dyn. 1989 Jun;6(6):1217-31. doi: 10.1080/07391102.1989.10506546.
8
Synthesis and physical characterization of DNA fragments containing N4-methylcytosine and 5-methylcytosine.含N4-甲基胞嘧啶和5-甲基胞嘧啶的DNA片段的合成与物理表征
Nucleic Acids Res. 1987 Oct 26;15(20):8467-78. doi: 10.1093/nar/15.20.8467.
9
10
Synthesis and conformational analysis of synthetic DNA fragments.合成DNA片段的合成与构象分析。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:403-9. doi: 10.1101/sqb.1983.047.01.047.
引用本文的文献
1
A Chemical Approach to Introduce 2,6-Diaminopurine and 2-Aminoadenine Conjugates into Oligonucleotides without Need for Protecting Groups.一种无需保护基团即可将 2,6-二氨基嘌呤和 2-氨基腺嘌呤缀合到寡核苷酸中的化学方法。
Org Lett. 2022 Aug 26;24(33):6111-6116. doi: 10.1021/acs.orglett.2c01848. Epub 2022 Aug 16.
2
Nearest-neighbor thermodynamics of deoxyinosine pairs in DNA duplexes.DNA双链体中脱氧肌苷对的近邻热力学
Nucleic Acids Res. 2005 Nov 1;33(19):6258-67. doi: 10.1093/nar/gki918. Print 2005.
3
Pyrazolo[3,4-d]pyrimidine nucleic acids: adjustment of dA-dT to dG-dC base pair stability.吡唑并[3,4-d]嘧啶核酸：dA-dT到dG-dC碱基对稳定性的调整。
Nucleic Acids Res. 2001 May 15;29(10):2069-78. doi: 10.1093/nar/29.10.2069.
4
Specifically alkylated DNA fragments. Synthesis and physical characterization of d[CGC(O6Me)GCG] and d[CGT(O6Me)GCG].特定烷基化的DNA片段。d[CGC(O6Me)GCG]和d[CGT(O6Me)GCG]的合成及物理特性表征
Nucleic Acids Res. 1983 May 25;11(10):3393-403. doi: 10.1093/nar/11.10.3393.
5
The B----Z transition in two synthetic oligonucleotides: d(C-2-amino-ACGTG) and d(m5CGCAm5CGTGCG) studied by IR, NMR and CD spectroscopies.通过红外光谱、核磁共振光谱和圆二色光谱研究的两种合成寡核苷酸：d(C-2-氨基-ACGTG) 和 d(m5CGCAm5CGTGCG) 中的B-Z转变
Nucleic Acids Res. 1984 Aug 10;12(15):6291-305. doi: 10.1093/nar/12.15.6291.
6
Structure of mouse rRNA precursors. Complete sequence and potential folding of the spacer regions between 18S and 28S rRNA.小鼠核糖体RNA前体的结构。18S与28S核糖体RNA之间间隔区的完整序列及潜在折叠。
Nucleic Acids Res. 1983 May 25;11(10):3375-91. doi: 10.1093/nar/11.10.3375.
7
Modified oligonucleotides as alternatives to the synthesis of mixed probes for the screening of cDNA libraries.修饰寡核苷酸作为合成混合探针用于筛选cDNA文库的替代方法。
Proc Natl Acad Sci U S A. 1985 Nov;82(22):7510-4. doi: 10.1073/pnas.82.22.7510.
8
B- to Z-DNA transition probed by oligonucleotides containing methylphosphonates.含甲基膦酸酯的寡核苷酸对B型到Z型DNA转变的探测
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1617-21. doi: 10.1073/pnas.83.6.1617.
9
Structural interconversion of alternating purine-pyrimidine inverted repeats cloned in supercoiled plasmids.克隆于超螺旋质粒中的交替嘌呤-嘧啶反向重复序列的结构相互转换。
Nucleic Acids Res. 1988 Jul 25;16(14B):6915-33. doi: 10.1093/nar/16.14.6915.
10
Thermodynamic studies of base pairing involving 2,6-diaminopurine.涉及2,6-二氨基嘌呤碱基配对的热力学研究。
Nucleic Acids Res. 1988 Jun 10;16(11):5115-22. doi: 10.1093/nar/16.11.5115.
本文引用的文献
1
Left-handed double helical DNA: variations in the backbone conformation.左手双螺旋DNA：主链构象的变化
Science. 1981 Jan 9;211(4478):171-6. doi: 10.1126/science.7444458.
2
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡脱氧核糖核苷酸的快速合成。IV. 通过磷酸三酯中间体改进寡脱氧核糖核苷酸的固相合成
Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081.
3
Salt-induced conformational changes of poly(dA-dT).盐诱导的聚（dA-dT）构象变化。
Nucleic Acids Res. 1980 Sep 11;8(17):3965-73. doi: 10.1093/nar/8.17.3965.
4
Structure of a B-DNA dodecamer. III. Geometry of hydration.B型DNA十二聚体的结构。III. 水合作用的几何学
J Mol Biol. 1981 Sep 25;151(3):535-56. doi: 10.1016/0022-2836(81)90009-7.
5
DNA and RNA oligomer thermodynamics: the effect of mismatched bases on double-helix stability.DNA和RNA寡聚物热力学：错配碱基对双螺旋稳定性的影响。
Biopolymers. 1981 Dec;20(12):2509-31. doi: 10.1002/bip.1981.360201204.
6
Changes in the helical repeat of poly(dG-m5dC) . poly(dG-m5dC) and poly(dG-dC) . poly(dG-dC) associated with the B-Z transition.与B-Z转变相关的聚（dG-m5dC）·聚（dG-m5dC）和聚（dG-dC）·聚（dG-dC）螺旋重复序列的变化。
Nature. 1981;293(5829):233-5. doi: 10.1038/293233a0.
7
Calorimetric and spectroscopic investigation of the helix-to-coil transition of the self-complementary deoxyribonucleotide ATGCAT.自互补脱氧核糖核苷酸ATGCAT螺旋-线圈转变的量热法和光谱法研究
Biophys Chem. 1981 Apr;13(2):141-9. doi: 10.1016/0301-4622(81)80013-0.
8
Conformational changes of poly(dG-dC) . poly(dG-dC) modified by the carcinogen N-acetoxy-N-acetyl-2-aminofluorene.经致癌物N-乙酰氧基-N-乙酰-2-氨基芴修饰的聚（dG-dC）·聚（dG-dC）的构象变化
Nucleic Acids Res. 1981 Mar 11;9(5):1241-50. doi: 10.1093/nar/9.5.1241.
9
Effect of tetramethylammonium ion on the helix-to-coil transition of poly(deoxyadenylylthymidine): a nuclear magnetic resonance and calorimetric investigation.四甲基铵离子对聚（脱氧腺苷酸-胸腺嘧啶）螺旋-卷曲转变的影响：核磁共振和量热研究
Biochemistry. 1981 Mar 17;20(6):1427-31. doi: 10.1021/bi00509a004.
10
The anatomy of A-, B-, and Z-DNA.A-DNA、B-DNA和Z-DNA的结构
Science. 1982 Apr 30;216(4545):475-85. doi: 10.1126/science.7071593.
Zotero 插件