Schwartz M P, Recker R R
Metab Bone Dis Relat Res. 1982;4(4):237-41. doi: 10.1016/0221-8747(82)90033-9.
Twenty healthy female beagles, all postbreeding age, were given two bone-labeling agents. Transilial bone biopsies were obtained and embedded undecalcified. Double-labeled, single-labeled, and unlabeled surfaces were determined along with the interlabel width on unstained sections under fluorescent light. Mean osteon thickness was determined on toluidine blue-stained sections. A mathematical model for predicting the amount of singly labeled surface was tested and compared to the amount of surface actually found occupied by single labels of either color. We found that the model accurately predicted the amount of singly labeled surface and that the amount of singly labeled surface labeled by one agent was equal to that labeled by the other (r = 0.977, P less than .001). We conclude that singly labeled surfaces in these animals were so labeled because bone formation either began or ended in the interval between administration of the two labels and that the proposed model accurately predicts this quantity of singly labeled surface without the need to measure any singly labeled surface. This label escape phenomenon must be taken into account when measuring bone formation rates by tetracycline labeling, and the model should be used whenever it is suspected that single labels appear that are not due to label escape.
二十只健康的雌性比格犬，均已过繁殖年龄，被给予两种骨标记剂。获取经皮骨活检标本并进行不脱钙包埋。在荧光灯下，在未染色的切片上测定双标记、单标记和未标记的表面以及标记间宽度。在甲苯胺蓝染色的切片上测定平均骨单位厚度。测试了一个预测单标记表面量的数学模型，并将其与实际发现的由任何一种颜色的单标记占据的表面量进行比较。我们发现该模型准确地预测了单标记表面的量，并且由一种试剂标记的单标记表面量与由另一种试剂标记的量相等（r = 0.977，P小于0.001）。我们得出结论，这些动物中的单标记表面之所以如此标记，是因为骨形成在两种标记给药的间隔期间开始或结束，并且所提出的模型无需测量任何单标记表面就能准确预测单标记表面的这一数量。在用四环素标记测量骨形成率时，必须考虑这种标记逃逸现象，并且每当怀疑出现不是由于标记逃逸导致的单标记时，都应使用该模型。
