Green K, Lindorme P S, Bowman K A, Huff J W, Alderman N
Curr Eye Res. 1982;2(11):791-6. doi: 10.3109/02713688209020013.
Either isolated normal or preswollen rabbit corneas were perfused across their endothelial surface with various drugs in the specular microscope. The deswelling rate of preswollen corneas was uninfluenced by calmodulin (1 and 10 micrograms/ml), phenylephrine (0.1 and 1 mM), DbcAMP (10(-4) M), theophylline (10(-3) and 10(-4) M), isoproterenol (2 x 10(-7) and 2 x 10(-6) M), or cyclic GMP (10(-4) and 10(-6) M). The swelling rate of normal thickness corneas was increased by furosemide (10(-5) and 10(-4) M) and thiocyanate (5 x 10(-2) and 5 x 10(-3) M) but not by SITS (10(-4) M), dipyridamole (5 x 10(-5) M) or NAP-taurine (10(-3) M). The results suggest that alteration of endothelial cyclic AMP or cyclic GMP levels has no influence on transendothelial fluid flow. Modulation of the metabolic processes underlying anion fluxes by furosemide and thiocyanate caused corneal swelling but none of the agents which affect passive anion exchange in other systems influenced transendothelial fluid movement. The furosemide and thiocyanate effects confirm that bicarbonate, or chloride, exchange may be important in part in the regulation of corneal dehydration by the endothelium.
在镜面显微镜下，用各种药物灌注分离出的正常兔角膜或预肿胀兔角膜的内皮表面。预肿胀角膜的消肿速率不受钙调蛋白（1和10微克/毫升）、去氧肾上腺素（0.1和1毫摩尔）、二丁酰环磷腺苷（10⁻⁴摩尔）、茶碱（10⁻³和10⁻⁴摩尔）、异丙肾上腺素（2×10⁻⁷和2×10⁻⁶摩尔）或环鸟苷酸（10⁻⁴和10⁻⁶摩尔）的影响。速尿（10⁻⁵和10⁻⁴摩尔）和硫氰酸盐（5×10⁻²和5×10⁻³摩尔）可增加正常厚度角膜的肿胀速率，但4-乙酰氨基-4’-异硫氰酸基芪-2,2’-二磺酸（SITS，10⁻⁴摩尔）、双嘧达莫（5×10⁻⁵摩尔）或N-乙酰半胱氨酸-牛磺酸（NAP-牛磺酸，10⁻³摩尔）则无此作用。结果表明，内皮细胞中环磷腺苷或环鸟苷酸水平的改变对跨内皮液体流动没有影响。速尿和硫氰酸盐对阴离子通量潜在代谢过程的调节导致角膜肿胀，但在其他系统中影响被动阴离子交换的药物均未影响跨内皮液体移动。速尿和硫氰酸盐的作用证实，碳酸氢根或氯离子交换可能在一定程度上对内皮细胞调节角膜脱水很重要。
